- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
Vimentin Antibody Phospho (pS82)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AJ1815e | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Vimentin Antibody Phospho (pS82) - Product info | |
| Application | WB
|
| Primary Accession | P08670 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP1071Y |
| Calculated MW | 53652 Da |
| Gene ID 7431 | |
| Target/Specificity A synthetic acetylated peptide corresponding to residues surrounding Ser82 of Vimentin was used as an immunogen. The antibody only detects Vimentin phosphorylated at Serine 82. | |
| Dilution WB~~1:10000~50000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Vimentin Antibody Phospho (pS82) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Vimentin Antibody Phospho (pS82) - Protein Information | |
| Name VIM | |
| Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally | |
| Cellular Location Cytoplasm. | |
| Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines | |
Vimentin Antibody Phospho (pS82) - Related products
AP2739a: Vimentin Antibody (S82)
AP2739b: Vimentin Antibody (C-term)
AP2739c: Vimentin Antibody (Center)
AP3546a: Phospho-Vimentin-S82 Antibody
LY10969a: VIM Over-expression Lysate
BP2739a: Vimentin Antibody (S82) Blocking Peptide
BP2739b: Vimentin Antibody (C-term) Blocking Peptide
BP2739c: Vimentin Antibody (Center) Blocking Peptide
BP3546a: Phospho-Vimentin-S82 Antibody Blocking Peptide
AT4513a: VIM Antibody (monoclonal) (M01)
AJ1815c: Vimentin Antibody Phospho (pS38)
Vimentin Antibody Phospho (pS82) - Application data
-
A. Western blot analysis on 1) AP treated and 2) untreated HeLa + Calyculin A cell lysate using anti-Vimentin (pS82) RabMab (Cat. #AJ1815e).
Vimentin Antibody Phospho (pS82) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed in during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
REFERENCES
1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992.
2. Inagaki M et al. Nature 328: 649-652, 1987.
3. Goto H et al. GENES CELLS 7:91-97. 2002.
4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998.
5. Yamaguchi T et al. JCB, 171(3):431-436, 2005.
