|Reactivity||Human, Mouse, Rat|
|Calculated MW||53652 Da|
|Other Names||Vimentin, VIM|
|Target/Specificity||A synthetic acetylated peptide corresponding to residues surrounding Ser82 of Vimentin was used as an immunogen. The antibody only detects Vimentin phosphorylated at Serine 82.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Vimentin Antibody Phospho (pS82) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed in during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992.
2. Inagaki M et al. Nature 328: 649-652, 1987.
3. Goto H et al. GENES CELLS 7:91-97. 2002.
4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998.
5. Yamaguchi T et al. JCB, 171(3):431-436, 2005.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.