|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||54306 Da|
|Other Names||Vitronectin, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, VTN|
|Target/Specificity||A synthetic peptide corresponding to residues near the V10 subunit of human vitronectin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Vitronectin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.|
|Cellular Location||Secreted, extracellular space.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Vitronectin, a 75 kDa polypeptide along with a 10 kDa fragment, is a glycoprotein present on most cell surfaces, in extracellular fluids and plasma (1). Vitronectin promotes cellular adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to several serpin serine protease inhibitors (2). As a member of the integrin family, Vitronectin contains the cell binding sequence arg gly asp (RGD), first found in fibronectin, and serves as a cell-to-substrate adhesion molecule (3). Vitronectin acts as an inhibitor of the complement cascade by binding to the C5b9 complex and it has also been shown to have has a novel and direct role in self-senescent-self intercellular recognition leading to macrophage phagocytosis of cells undergoing apoptosis (4).
1. Rouslahti E, et al. J Invest Dermatol 79:65-68, 1982
2. Bauer JS, et al. J Cell Biol. 116(2):477-87, 1992
3. Stefansson S, et al. Nature 383(6599):390-1, 1996
4. Savill J, et al. Nature 343:170-3, 1990
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.