|Calculated MW||49188 Da|
|Other Names||Wilms tumor protein, WT33, WT1|
|Target/Specificity||A recombinant protein corresponding to the N-terminus of human WT was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Wilm's Tumor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm Isoform 4: Nucleus, nucleoplasm.|
|Tissue Location||Expressed in the kidney and a subset of hematopoietic cells|
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Provided below are standard protocols that you may find useful for product applications.
Wilms Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilms tumors and highly expressed in a wide variety of other malignancies (1). WT1 consists of two major isoforms; WT1 (-KTS), a transcription factor, and WT1 (+KTS) (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome (3).
1. Spraggon L, et al. Oncogene. 2006, Aug 21. 2. Markus MA, et al. Exp Cell Res. 2006 Jul 25 3. Niaudet P, et al. Pediatr Nephrol. 2006 Aug 23
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