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YAP65 Antibody Phospho (pS127)

Rabbit Monoclonal Antibody

  • WB - YAP65 Antibody Phospho (pS127) AJ1822b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-YAP65 (pS127) RabMAb (Cat. #AJ1822b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with FBS + calyculin A.
  • IHC - YAP65 Antibody Phospho (pS127) AJ1822b
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-YAP65 RabMAb (Cat. #AJ1822b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P46937
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1675Y
Calculated MW 54462 Da
Gene ID 10413
Other Names Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1, YAP65
Target/Specificity A phospho specific peptide corresponding to residues surrounding Ser127 of human YAP65 was used as immunogen. The antibody detects YAP65 phosphorylated at Serine 127.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsYAP65 Antibody Phospho (pS127) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YAP1
Synonyms YAP65
Function Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).
Cellular Location Cytoplasm. Nucleus. Note=Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271).
Tissue Location Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Research Areas
Citations (0)

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The transcriptional coactivator Yes-associated protein (YAP, YAP65) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). YAP binds with 14-3-3 after being phosphorylated by Akt at Ser127. Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2).


1. Strano S, et al. Mol Cell 18(4):447-59, 2005
2. Basu S, et al. Mol Cell 11(1):11-23, 20033.

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Cat# AJ1822b
(40 western blots)
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