|Calculated MW||35924 Da|
|Other Names||Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1, NSEP1, YB1|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human YBOX1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||YBOX1 (YB-1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro.|
|Cellular Location||Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress|
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Provided below are standard protocols that you may find useful for product applications.
Human DNA-binding proteins, dbpA and dbpB (YBOX-1), are members of a protein family containing a cold-shock domain, and are regarded as transcriptional regulators (1). The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and TATA box. The Y box contains an inverted CCAAT box. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor (2). Overexpression of YB-1 resulted in increased levels of PTP1B. Furthermore, depletion of YB-1 protein, by expression of a specific antisense construct, led to an approximately 70% decrease in expression of PTP1B, but no change in the level of its closest relative, TC-PTP. Data reveal an important role for YB-1 as a regulator of PTP1B expression, and further highlight PTP1B as a critical regulator of insulin- and cytokine-mediated signal transduction (3).
1. Kudo S, et al. Eur J Biochem 231(1):72-82, 1995
2. Didier DK, et al. Proc Natl Acad Sci 85(19):7322-6, 1988
3. Fukada T, et al. EMBO J 22(3):479-93, 2003
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