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ZAP-70 Antibody Phospho (pY492)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1824d | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ZAP-70 Antibody Phospho (pY492) - Product info | |
| Application | WB
|
| Primary Accession | P43403 |
| Reactivity | Human |
| Clone Names | EP2291Y |
| Calculated MW | 69872 Da |
| Gene ID 7535 | |
| Other Names ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-associated protein;Syk-related tyrosine kinase | |
| Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr492 of human ZAP-70 was used as immunogen. The antibody only detects ZAP-70 phosphorylated on Tyrosine 492. | |
| Dilution WB~~1:1000~10000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ZAP-70 Antibody Phospho (pY492) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ZAP-70 Antibody Phospho (pY492) - Protein Information | |
| Name ZAP70 | |
| Synonyms SRK | |
| Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2 Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR) | |
| Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity) | |
| Tissue Location Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells | |
ZAP-70 Antibody Phospho (pY492) - Related products
AP3676a: Phospho-ZAP70-Y292 Antibody
AP7824c: ZAP70 Antibody (Center)
RI15782: ZAP70 predesign siRNA
BP3658a: Phospho-ZAP70-Y319 Blocking Peptide
BP3659a: Phospho-ZAP70-Y492 Blocking Peptide
BP3660a: Phospho-ZAP70-Y493 Blocking Peptide
BP3661a: Phospho-ErbB2-pY1248(M) Blocking Peptide
BP3676a: Phospho-ZAP70-Y292 Antibody Blocking Peptide
BP7824c: ZAP70 Antibody (Center) Blocking Peptide
AJ1824b: ZAP-70 Antibody (C-term)
ZAP-70 Antibody Phospho (pY492) - Application data
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A. Western blot analysis on Jurkat cell lysates using anti-Phospho-ZAP-70 (p492) RabMAb (Cat. #AJ1824d). Cells were either (A) untreated (B) treated with pervanadate
ZAP-70 Antibody Phospho (pY492) - Research Areas
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BACKGROUND
Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Lck phosphorylates Tyrosine 492 and 493 located at the activation loop of the catalytic domain of ZAP-70, leading to its activation and increased autophosphorylation (3).
REFERENCES
1. Chu, D.H., et al. Immunol. Rev. 165:167
2. Isakov, N., et al. J Exp Med. 181:375
3. Isakov, N., et al. J Exp Med. 181:375
