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>   home   >   Products   >   Primary Antibodies   >   Developmental Biology   >   NR5A1 / SF1 Antibody (Internal)   

NR5A1 / SF1 Antibody (Internal)

Rabbit Polyclonal Antibody

     
  • IHC - NR5A1 / SF1 Antibody (Internal) ALS10551
    Anti-NR5A1 antibody ALS10551 IHC of human brain, pituitary.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P
Primary Accession Q13285
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 52kDa
Dilution IHC-P (15-30 µg/ml)
Additional Information
Gene ID 2516
Other Names Steroidogenic factor 1, SF-1, STF-1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP, NR5A1, AD4BP, FTZF1, SF1
Target/Specificity Human NR5A1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Reconstitution & Storage Long term: -70°C; Short term: +4°C
PrecautionsNR5A1 / SF1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR5A1
Synonyms AD4BP, FTZF1, SF1
Function Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO- NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:11479297}
Tissue Location High expressed in the adrenal cortex, the ovary, the testis, and the spleen (PubMed:9177385)
Volume 50 µl
Research Areas
Citations (0)

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Background

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO- NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

References

Santa Barbara P.,et al.Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
Oba K.,et al.Biochem. Biophys. Res. Commun. 226:261-267(1996).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Yeh J.R.,et al.Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.

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$ 395.00
Cat# ALS10551
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