|Dilution||IHC-P (2-4 µg/ml)|
|Other Names||Mas-related G-protein coupled receptor member X2, MRGPRX2, MRGX2|
|Target/Specificity||Human MRGPRX2 / MRGX2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MRGPRX1 (71%), MRGPRX4 (64%), MRGPRX3 (57%).|
|Reconstitution & Storage||Long term: -70°C; Short term: +4°C|
|Precautions||MRGPRX2 / MRGX2 Antibody (Extracellular Domain) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mast cell-specific receptor for basic secretagogues (PubMed:25517090). Basic secretagogues are a set of cationic amphiphilic drugs, as well as endo- and exogenous peptides, which share basic head group combined with a hydrophobic core of the molecule. Recognizes and binds small molecules containing a cyclized a tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium (By similarity). Mediates mast cell responsiveness and side effects of small-molecule therapeutic drugs by acting as a specific receptor for basic secretagogues drugs in mast cells: binding to drugs induces pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction (By similarity). Acts as a receptor for a number of ligands, including peptides: acts as a receptor of cortistatin-14, a regulator of sleep regulation locomotor activity, and cortical function (PubMed:12915402). Acts as a receptor for proadrenomedullin N- terminal peptides PAMP-12, and at lower extent PAMP-20 (PubMed:15823563). Acts as a receptor for antibacterial protein LL-37, promoting chemotaxis, degranulation and chemokine production in mast cells (PubMed:22069323). Acts as a receptor for PMX-53 peptide, a potent antagonist of C5AR1/CD88 (PubMed:21441599). Acts as a receptor for beta-defensins (PubMed:23698749). Acts as a receptor for complanadine A, an alkaloid (PubMed:24930830).|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Mainly expressed in mast cells. Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion (PubMed:12915402). Detected in blood vessels, scattered lymphocytes, and gastrointestinal ganglia (at protein level) (PubMed:16161007).|
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Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin- stimulated cAMP levels, suggesting that this receptor is G(q)- coupled.
Dong X.,et al.Cell 106:619-632(2001).
Takeda S.,et al.FEBS Lett. 520:97-101(2002).
Suwa M.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Yang S.,et al.Gene 352:30-35(2005).
Martin A.L.,et al.Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
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