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NEK7 Antibody (Kinase Domain)

Rabbit Polyclonal Antibody

     
  • IHC - NEK7 Antibody (Kinase Domain) ALS10888
    Anti-NEK7 antibody ALS10888 IHC of human brain, neurons and glia.
    detail
  • IHC - NEK7 Antibody (Kinase Domain) ALS10888
    Anti-NEK7 antibody IHC of human Colon, Carcinoma.
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P
Primary Accession Q8TDX7
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 35kDa
Dilution IHC-P (1 µg/ml)
Additional Information
Gene ID 140609
Other Names Serine/threonine-protein kinase Nek7, 2.7.11.1, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7
Target/Specificity Human NEK7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Reconstitution & Storage Long term: -70°C; Short term: +4°C
PrecautionsNEK7 Antibody (Kinase Domain) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEK7 {ECO:0000303|PubMed:11701951, ECO:0000312|HGNC:HGNC:13386}
Function Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:31409757, PubMed:19941817). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:31409757, PubMed:19941817, PubMed:26522158). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q9ES74}. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity). {ECO:0000250|UniProtKB:Q9ES74, ECO:0000269|PubMed:17586473}
Tissue Location Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

References

Kimura M.,et al.Cytogenet. Cell Genet. 94:33-38(2001).
Melton D.,et al.Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Belham C.,et al.J. Biol. Chem. 278:34897-34909(2003).

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$ 467.50
Cat# ALS10888
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