|Application ||WB, IHC-P, IF, ICC|
|Dilution||ICC (10 µg/ml), IHC-P (5 µg/ml), WB (1-2 µg/ml)|
|Other Names||Single-stranded DNA cytosine deaminase, 18.104.22.168, Activation-induced cytidine deaminase, Cytidine aminohydrolase, AICDA, AID|
|Target/Specificity||peptide corresponding to 14 amino acids near the carboxy-terminus of human AID|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||AICDA / AID Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses (PubMed:18722174, PubMed:21385873, PubMed:21518874, PubMed:27716525). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (PubMed:21496894).|
|Cellular Location||Nucleus. Cytoplasm Note=Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm.|
|Tissue Location||Strongly expressed in lymph nodes and tonsils.|
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Provided below are standard protocols that you may find useful for product applications.
Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.
Muto T.,et al.Genomics 68:85-88(2000).
Revy P.,et al.Cell 102:565-575(2000).
Martin A.,et al.Proc. Natl. Acad. Sci. U.S.A. 99:12304-12308(2002).
Roa S.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
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