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AGAP2 / PIKE Antibody (C-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q99490 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 125kDa |
| Dilution | IHC-P (20 µg/ml), WB (0.5-2 µg/ml), |
| Gene ID | 116986 |
|---|---|
| Other Names | Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, AGAP-2, Centaurin-gamma-1, Cnt-g1, GTP-binding and GTPase-activating protein 2, GGAP2, Phosphatidylinositol 3-kinase enhancer, PIKE, AGAP2, CENTG1, KIAA0167 |
| Target/Specificity | Anti-PIKE antibody will detect both PIKE-L and PIKE-A isoforms. |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
| Precautions | AGAP2 / PIKE Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AGAP2 |
|---|---|
| Synonyms | CENTG1, KIAA0167 |
| Function | GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. |
| Cellular Location | [Isoform 1]: Cytoplasm. Nucleus. |
| Tissue Location | Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart |
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Provided below are standard protocols that you may find useful for product applications.
Background
GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
References
Elkahloun A.G.,et al.Genomics 42:295-301(1997).
Roe B.,et al.Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
Xia C.,et al.Mol. Cell. Biol. 23:2476-2488(2003).
Rong R.,et al.Nat. Neurosci. 6:1153-1161(2003).
Hong W.,et al.Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
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