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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   BIRC5 / Survivin Antibody (C-Terminus)   

BIRC5 / Survivin Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • ICC - BIRC5 / Survivin Antibody (C-Terminus) ALS11280
    Immunocytochemistry staining of MOLT4 cells using survivin at 10 ug/ml.
  • IF - BIRC5 / Survivin Antibody (C-Terminus) ALS11280
    Immunofluorescence of Survivin in Molt4 cells with Survivin antibody at 10 ug/ml.
  • WB - BIRC5 / Survivin Antibody (C-Terminus) ALS11280
    Western blot of survivin in MOLT4 cell lysate in the absence (A) or presence (B) of blocking...
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession O15392
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 16kDa
Dilution IHC-P (2.5 µg/ml), WB (0.5-1 µg/ml),
Additional Information
Gene ID 332
Other Names Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, API4, IAP4
Target/Specificity peptide corresponding to amino acids near the carboxy-terminus of human survivin
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsBIRC5 / Survivin Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BIRC5
Synonyms API4, IAP4
Function Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti- apoptotic effects when compared with the displayed wild-type isoform.
Cellular Location Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.
Tissue Location Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Research Areas
Citations (0)

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Background

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

References

Ambrosini G.,et al.Nat. Med. 3:917-921(1997).
Mahotka C.,et al.Cancer Res. 59:6097-6102(1999).
Uren A.G.,et al.Curr. Biol. 10:1319-1328(2000).
Badran A.,et al.Biochem. Biophys. Res. Commun. 314:902-907(2004).
Zheng W.,et al.DNA Seq. 16:321-328(2005).

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$ 395.00
Cat# ALS11280
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