|Application ||WB, IHC-P, IF, E|
|Dilution||ELISA (1:2000-1:8000), IHC-P (2.5 µg/ml), WB (1:500-1:2000)|
|Other Names||AP-1 complex subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Adaptor-related protein complex 1 subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, Gamma1-adaptin, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, AP1G1, ADTG, CLAPG1|
|Target/Specificity||aa 646-659 of Human AP1G1.|
|Reconstitution & Storage||+4°C or -20°C, Avoid repeated freezing and thawing.|
|Precautions||AP1G1 / Adaptin Gamma 1 Antibody (aa646-659) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.|
|Cellular Location||Golgi apparatus. Cytoplasmic vesicle, clathrin- coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Peyrard M.,et al.Genomics 50:275-280(1998).
Takatsu H.,et al.J. Biol. Chem. 273:24693-24700(1998).
Martin J.,et al.Nature 432:988-994(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Deneka M.,et al.EMBO J. 22:2645-2657(2003).
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