|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat, Pig, Dog|
|Dilution||ELISA (1:20000-1:100000), IHC-P (1:500), WB (1:500-1:2000)|
|Other Names||Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN|
|Target/Specificity||Synthetic peptide C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.|
|Precautions||SPP1 / Osteopontin Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.|
|Tissue Location||Bone. Found in plasma.|
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Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Kiefer M.C.,et al.Nucleic Acids Res. 17:3306-3306(1989).
Young M.F.,et al.Genomics 7:491-502(1990).
Shiraga H.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992).
Crosby A.H.,et al.Genomics 27:155-160(1995).
Hijiya N.,et al.Biochem. J. 303:255-262(1994).
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