|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat, Pig, Dog|
|Dilution||ELISA (1:20000-1:100000), IHC-P (1:500), WB (1:500-1:2000)|
|Other Names||Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN|
|Target/Specificity||Synthetic peptide C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.|
|Precautions||SPP1 / Osteopontin Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.|
|Tissue Location||Bone. Found in plasma.|
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Provided below are standard protocols that you may find useful for product applications.
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Kiefer M.C.,et al.Nucleic Acids Res. 17:3306-3306(1989).
Young M.F.,et al.Genomics 7:491-502(1990).
Shiraga H.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992).
Crosby A.H.,et al.Genomics 27:155-160(1995).
Hijiya N.,et al.Biochem. J. 303:255-262(1994).
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