RYBP Antibody (aa215-228)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF |
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Primary Accession | Q8N488 |
Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Horse, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 25kDa |
Dilution | IHC-P (2.5 µg/ml), WB (0.5-1 µg/ml), |
Gene ID | 23429 |
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Other Names | RING1 and YY1-binding protein, Apoptin-associating protein 1, APAP-1, Death effector domain-associated factor, DED-associated factor, YY1 and E4TF1-associated factor 1, RYBP, DEDAF, YEAF1 |
Target/Specificity | synthetic peptide (TPKGDMSAVNDESF) corresponding to amino acids 215 to 228 of human DEDAF The sequence is identical to that of mouse origin |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
Precautions | RYBP Antibody (aa215-228) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RYBP |
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Synonyms | DEDAF, YEAF1 |
Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911). |
Cellular Location | Nucleus. Cytoplasm. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q8CCI5}. Note=Primarily found in the nucleus Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies (By similarity). {ECO:0000250|UniProtKB:Q8CCI5} |
Tissue Location | Down-regulated in breast cancer tissues and in several breast cancer cell lines (at protein level) (PubMed:27748911) Widely expressed with highest levels in lymphoid tissues and placenta |
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Background
Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.
References
Zheng L.,et al.J. Biol. Chem. 276:31945-31952(2001).
Sawa C.,et al.J. Biol. Chem. 277:22484-22490(2002).
Danen-van Oorschot A.A.M.M.,et al.Cell Death Differ. 11:564-573(2004).
Cheng C.M.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Gearhart M.D.,et al.Mol. Cell. Biol. 26:6880-6889(2006).
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