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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RYBP Antibody (aa215-228)   

RYBP Antibody (aa215-228)

Rabbit Polyclonal Antibody

     
  • IF - RYBP Antibody (aa215-228) ALS11437
    Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 ug/ml.
  • WB - RYBP Antibody (aa215-228) ALS11437
    Western blot of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C)...
  • IHC - RYBP Antibody (aa215-228) ALS11437
    Anti-RYBP antibody IHC of human placenta.
  • IHC - RYBP Antibody (aa215-228) ALS11437
    Anti-RYBP antibody IHC of human spleen.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession Q8N488
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Horse, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 25kDa
Dilution IHC-P (2.5 µg/ml), WB (0.5-1 µg/ml),
Additional Information
Gene ID 23429
Other Names RING1 and YY1-binding protein, Apoptin-associating protein 1, APAP-1, Death effector domain-associated factor, DED-associated factor, YY1 and E4TF1-associated factor 1, RYBP, DEDAF, YEAF1
Target/Specificity synthetic peptide (TPKGDMSAVNDESF) corresponding to amino acids 215 to 228 of human DEDAF The sequence is identical to that of mouse origin
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsRYBP Antibody (aa215-228) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RYBP
Synonyms DEDAF, YEAF1
Function Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity).
Cellular Location Nucleus. Cytoplasm Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q8CCI5} Note=Primarily found in the nucleus. Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies (By similarity). {ECO:0000250|UniProtKB:Q8CCI5}
Tissue Location Widely expressed with highest levels in lymphoid tissues and placenta.
Research Areas
Citations (0)

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Background

Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.

References

Zheng L.,et al.J. Biol. Chem. 276:31945-31952(2001).
Sawa C.,et al.J. Biol. Chem. 277:22484-22490(2002).
Danen-van Oorschot A.A.M.M.,et al.Cell Death Differ. 11:564-573(2004).
Cheng C.M.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Gearhart M.D.,et al.Mol. Cell. Biol. 26:6880-6889(2006).

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$ 425.00
Cat# ALS11437
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