ICSBP / IRF8 Antibody (C-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF |
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Primary Accession | Q02556 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 48kDa |
Dilution | IHC-P (5 µg/ml), WB (1-2 µg/ml), |
Gene ID | 3394 |
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Other Names | Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, H-ICSBP, ICSBP, IRF8, ICSBP1 |
Target/Specificity | peptide corresponding to 16 amino acids near the carboxy-terminus of human IRF8 |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
Precautions | ICSBP / IRF8 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | IRF8 {ECO:0000303|PubMed:21524210, ECO:0000312|HGNC:HGNC:5358} |
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Function | Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed:25122610). Can both act as a transcriptional activator or repressor (By similarity). Plays a negative regulatory role in cells of the immune system (By similarity). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA- 3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity). Positively regulates macroautophagy in dendritic cells (PubMed:29434592). Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity). |
Cellular Location | Nucleus. Cytoplasm Note=In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction. |
Tissue Location | Predominantly expressed in lymphoid tissues. |
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Background
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity).
References
Weisz A.,et al.J. Biol. Chem. 267:25589-25596(1992).
Schmidt M.,et al.Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
Cohen H.,et al.J. Biol. Chem. 275:39081-39089(2000).
Basso K.,et al.J. Exp. Med. 209:2455-2465(2012).
Sjoeblom T.,et al.Science 314:268-274(2006).
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