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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   TRIM5 Antibody   

TRIM5 Antibody

Rabbit Polyclonal Antibody

     
  • IF - TRIM5 Antibody ALS11500
    Immunofluorescence of TRIM5 alpha in Human Stomach cells with TRIM5 alpha antibody at 10 ug/ml.
  • WB - TRIM5 Antibody ALS11500
    Western blot of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell...
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession Q9C035
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 56kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 85363
Other Names Tripartite motif-containing protein 5, 6.3.2.-, RING finger protein 88, TRIM5, RNF88
Target/Specificity synthetic peptide corresponding to amino acids near the mid-region of human TRIM5a
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsTRIM5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM5
Synonyms RNF88
Function Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF- kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV).
Cellular Location Cytoplasm, P-body Note=Closely associates with proteasomal subunits in cytoplasmic bodies (By similarity). Colocalizes with SQSTM1 in cytoplasmic bodies.
Research Areas
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Background

Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF- kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV).

References

Reymond A.,et al.EMBO J. 20:2140-2151(2001).
Yap M.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:10786-10791(2004).
Sawyer S.L.,et al.Curr. Biol. 16:95-100(2006).
Yamauchi K.,et al.FEBS J. 275:1540-1555(2008).
Battivelli E.,et al.J. Virol. 85:7828-7835(2011).

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$ 395.00
Cat# ALS11500
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