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>   home   >   Products   >   Primary Antibodies   >   DNMT / DNMT1 Antibody (aa177-550)   

DNMT / DNMT1 Antibody (aa177-550)

Chicken Polyclonal Antibody

     
  • IHC - DNMT / DNMT1 Antibody (aa177-550) ALS11543
    Anti-DNMT1 antibody IHC of human kidney.
  • IHC - DNMT / DNMT1 Antibody (aa177-550) ALS11543
    Anti-DNMT1 antibody IHC of human placenta.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P26358
Reactivity Human
Host Chicken
Clonality Polyclonal
Calculated MW 183kDa
Dilution IHC-P (2.5 µg/ml), WB (1:2000),
Additional Information
Gene ID 1786
Other Names DNA (cytosine-5)-methyltransferase 1, Dnmt1, 2.1.1.37, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT, DNMT1, AIM, CXXC9, DNMT
Target/Specificity Amino acids 177-550 human DNMT1
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsDNMT / DNMT1 Antibody (aa177-550) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT1
Synonyms AIM, CXXC9, DNMT
Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Cellular Location Nucleus.
Tissue Location Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Citations (0)

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Background

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

References

Yen R.-W.C.,et al.Nucleic Acids Res. 20:2287-2291(1992).
Yoder J.A.,et al.J. Biol. Chem. 271:31092-31097(1996).
Li L.C.,et al.Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Hsu D.-W.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:9751-9756(1999).

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$ 395.00
Cat# ALS11543
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