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SUPT16H / FACTP140 Antibody (aa321-640)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IP |
|---|---|
| Primary Accession | Q9Y5B9 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 120kDa |
| Dilution | IHC-P (20 µg/ml) |
| Gene ID | 11198 |
|---|---|
| Other Names | FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chromatin transcription complex subunit SPT16, hSPT16, SUPT16H, FACT140, FACTP140 |
| Target/Specificity | Recombinant protein comprised of GST and amino acids 321-640 of human SUPT16H |
| Reconstitution & Storage | Store at -20°C. Aliquot to avoid freeze/thaw cycles. |
| Precautions | SUPT16H / FACTP140 Antibody (aa321-640) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SUPT16H |
|---|---|
| Synonyms | FACT140, FACTP140 |
| Function | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). |
| Cellular Location | Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci |
| Tissue Location | Ubiquitous.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene.
References
Orphanides G.,et al.Nature 400:284-288(1999).
Bienvenut W.V.,et al.Submitted (JUL-2007) to UniProtKB.
Bienvenut W.V.,et al.Submitted (MAR-2008) to UniProtKB.
Kang S.-W.,et al.Genes Cells 5:251-263(2000).
Orphanides G.,et al.Cell 92:105-116(1998).
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