|Dilution||IHC-P (5 µg/ml)|
|Other Names||T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL|
|Target/Specificity||Peptide corresponding to the C-terminal amino acids of the cytoplasmic domain of human CD8 alpha chain|
|Reconstitution & Storage||+4°C, avoid freezing|
|Precautions||CD8A / CD8 Alpha Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK- cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein Note=CD8A localizes to lipid rafts only when associated with its partner CD8B.|
|Tissue Location||CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and dendritic cells expresses CD8A homo-dimers Expressed at the cell surface of plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Littman D.R.,et al.Cell 40:237-246(1985).
Parnes J.R.,et al.Behring Inst. Mitt. 77:48-55(1985).
Sukhatme V.P.,et al.Cell 40:591-597(1985).
Nakayama K.,et al.Immunogenetics 30:393-397(1989).
Norment A.M.,et al.J. Immunol. 142:3312-3319(1989).
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