|Application ||WB, IHC-P, IF, E|
|Dilution||ELISA (1:10000-1:50000), IHC-P (5 µg/ml), WB (1:500-1:2000)|
|Other Names||Ubl carboxyl-terminal hydrolase 18, 3.4.19.-, 43 kDa ISG15-specific protease, hUBP43, ISG15-specific-processing protease, Ubl thioesterase 18, USP18, ISG43|
|Target/Specificity||Amino acids 40 to 49 of human USP18|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||UBP43 / USP18 Antibody (aa40-49) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.|
|Cellular Location||Isoform 1: Cytoplasm.|
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Provided below are standard protocols that you may find useful for product applications.
Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
Li X.-L.,et al.Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
Schwer H.,et al.Genomics 65:44-52(2000).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
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