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>   home   >   Products   >   Primary Antibodies   >   H2AFX / H2AX Antibody (Ser139)   

H2AFX / H2AX Antibody (Ser139)

Rabbit Polyclonal Antibody

     
  • IF - H2AFX / H2AX Antibody (Ser139) ALS11769
    Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X(Ab-139) antibody.
  • IHC - H2AFX / H2AX Antibody (Ser139) ALS11769
    Anti-H2AX antibody IHC of human colon.
  • IHC - H2AFX / H2AX Antibody (Ser139) ALS11769
    Anti-H2AX antibody IHC of human testis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P16104
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 15kDa
Dilution IHC-P (10 µg/ml), WB (1:500-1:1000),
Additional Information
Gene ID 3014
Other Names Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX
Target/Specificity Amino acids surrounding Ser 139 of human H2AFX
Reconstitution & Storage +4°C or -20°C, Avoid repeated freezing and thawing.
PrecautionsH2AFX / H2AX Antibody (Ser139) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2AFX
Synonyms H2AX
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
Cellular Location Nucleus Chromosome
Citations (0)

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Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.

References

Mannironi C.,et al.Nucleic Acids Res. 17:9113-9126(1989).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Rogakou E.P.,et al.J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P.,et al.J. Cell Biol. 146:905-916(1999).
Paull T.T.,et al.Curr. Biol. 10:886-895(2000).

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$ 395.00
Cat# ALS11769
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