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>   home   >   Products   >   Primary Antibodies   >   FEN1 Antibody (aa1-380, clone 4E7)   

FEN1 Antibody (aa1-380, clone 4E7)

Mouse Monoclonal Antibody

     
  • IHC - FEN1 Antibody (aa1-380, clone 4E7) ALS11857
    Anti-FEN1 antibody IHC of human brain, cortex.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P39748
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4E7
Calculated MW 43kDa
Dilution IHC-P (5 µg/ml)
Additional Information
Gene ID 2237
Other Names Flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}, FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140}, 3.1.-.- {ECO:0000255|HAMAP-Rule:MF_03140}, DNase IV, Flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}, Maturation factor 1, MF1, hFEN-1, FEN1 {ECO:0000255|HAMAP-Rule:MF_03140}, RAD2
Target/Specificity Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsFEN1 Antibody (aa1-380, clone 4E7) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FEN1 {ECO:0000255|HAMAP-Rule:MF_03140}
Synonyms RAD2
Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Cellular Location Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage
Volume 50 µl
Citations (0)

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Background

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

References

Murray J.M.,et al.Mol. Cell. Biol. 14:4878-4888(1994).
Hiraoka L.R.,et al.Genomics 25:220-225(1995).
Taylor T.D.,et al.Nature 440:497-500(2006).
Robins P.,et al.J. Biol. Chem. 269:28535-28538(1994).
Shen B.,et al.J. Biol. Chem. 271:9173-9176(1996).

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$ 395.00
Cat# ALS11857
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