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>   home   >   Products   >   Primary Antibodies   >   RAD1 Antibody (N-Terminus, clone 4126)   

RAD1 Antibody (N-Terminus, clone 4126)

Mouse Monoclonal Antibody

     
  • IHC - RAD1 Antibody (N-Terminus, clone 4126) ALS11866
    Anti-RAD1 antibody IHC of human testis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession O60671
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4126
Calculated MW 32kDa
Dilution IHC-P (10 µg/ml)
Additional Information
Gene ID 5810
Other Names Cell cycle checkpoint protein RAD1, hRAD1, 3.1.11.2, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1
Target/Specificity Amino terminal fragment of human Rad1 purified from E. coli.
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsRAD1 Antibody (N-Terminus, clone 4126) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD1
Synonyms REC1
Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Cellular Location Nucleus.
Tissue Location Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level)
Citations (0)

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Background

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

References

Freire R.,et al.Genes Dev. 12:2560-2573(1998).
Bluyssen H.A.R.,et al.Genomics 54:331-337(1998).
Marathi U.K.,et al.Genomics 54:344-347(1998).
Dean F.B.,et al.Genomics 54:424-436(1998).
Parker A.E.,et al.J. Biol. Chem. 273:18332-18339(1998).

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$ 395.00
Cat# ALS11866
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