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RNF213 Antibody (Internal)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, E |
|---|---|
| Primary Accession | Q63HN8 |
| Reactivity | Human |
| Host | Goat |
| Clonality | Polyclonal |
| Calculated MW | 591kDa |
| Dilution | ELISA (1:128000), IHC-P (1.25 µg/ml), |
| Gene ID | 57674 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RNF213, 6.3.2.-, ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RNF213, ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR |
| Target/Specificity | Human RNF213. |
| Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
| Precautions | RNF213 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF213 (HGNC:14539) |
|---|---|
| Function | Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PubMed:21799892, PubMed:26126547, PubMed:26278786, PubMed:26766444, PubMed:30705059, PubMed:32139119, PubMed:34012115). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc- finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (PubMed:34012115). Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains (PubMed:34012115). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (PubMed:34012115). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (PubMed:30705059). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (PubMed:30846318). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys-63'-linked ubiquitination of target proteins (PubMed:32139119, PubMed:33842849). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (PubMed:26766444). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (PubMed:34012115). |
| Cellular Location | Cytoplasm, cytosol. Lipid droplet |
| Tissue Location | Widely expressed (at protein level). [Isoform 2]: Minor isoform with restricted expression. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Probable E3 ubiquitin-protein ligase that may play a role in angiogenesis. May also have an ATPase activity.
References
Liu W.,et al.PLoS ONE 6:E22542-E22542(2011).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Cools J.,et al.Genes Chromosomes Cancer 34:354-362(2002).
Nagase T.,et al.DNA Res. 7:273-281(2000).
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