HIPK3 / FIST Antibody (aa850-900)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
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Primary Accession | Q9H422 |
Reactivity | Human, Mouse, Rat, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 134kDa |
Dilution | IHC-P (10 µg/ml), WB (0.5-2 µg/ml), |
Gene ID | 10114 |
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Other Names | Homeodomain-interacting protein kinase 3, 2.7.11.1, Androgen receptor-interacting nuclear protein kinase, ANPK, Fas-interacting serine/threonine-protein kinase, FIST, Homolog of protein kinase YAK1, HIPK3, DYRK6, FIST3, PKY |
Target/Specificity | A portion of amino acids 850-900 of human HIPK3 |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | HIPK3 / FIST Antibody (aa850-900) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HIPK3 |
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Synonyms | DYRK6, FIST3, PKY |
Function | Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2- mediated activation of the osteocalcin FGF-responsive element (OCFRE). |
Cellular Location | Cytoplasm. Nucleus |
Tissue Location | Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung |
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Provided below are standard protocols that you may find useful for product applications.
Background
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF- responsive element (OCFRE).
References
Begley D.A.,et al.Gene 200:35-43(1997).
Rochat-Steiner V.,et al.J. Exp. Med. 192:1165-1174(2000).
Taylor T.D.,et al.Nature 440:497-500(2006).
Becker W.,et al.Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
Moilanen A.-M.,et al.Mol. Biol. Cell 9:2527-2543(1998).
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