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CD9 Antibody (clone HI9a)

Mouse Monoclonal Antibody

     
  • IHC - CD9 Antibody (clone HI9a) ALS12028
    Anti-CD9 antibody IHC of human skin.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, Flo
Primary Accession P21926
Reactivity Human, Rabbit, Sheep, Horse, Bovine, Dog
Host Mouse
Clonality Monoclonal
Clone Names HI9a
Calculated MW 25kDa
Dilution Flo (0.5 µg/10E6 cells), IHC-P (10 µg/ml),
Additional Information
Gene ID 928
Other Names CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD9, CD9, MIC3, TSPAN29
Reconstitution & Storage Store undiluted at 4 degrees C
PrecautionsCD9 Antibody (clone HI9a) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD9 {ECO:0000303|PubMed:1840589, ECO:0000312|HGNC:HGNC:1709}
Function Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626).
Cellular Location Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Secreted, extracellular exosome {ECO:0000250|UniProtKB:P40240}. Note=Present at the cell surface of oocytes. Accumulates in the adhesion area between the sperm and egg following interaction between IZUMO1 and its receptor IZUMO1R/JUNO {ECO:0000250|UniProtKB:P40240}
Tissue Location Detected in platelets (at protein level) (PubMed:19640571). Expressed by a variety of hematopoietic and epithelial cells (PubMed:19640571).
Research Areas
Citations (0)
citation

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Background

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.

References

Boucheix C.,et al.J. Biol. Chem. 266:117-122(1991).
Lanza F.,et al.J. Biol. Chem. 266:10638-10645(1991).
Miyake M.,et al.J. Exp. Med. 174:1347-1354(1991).
Rubinstein E.,et al.Genomics 16:132-138(1993).
Kobayashi H.,et al.Clin. Exp. Immunol. 137:101-108(2004).

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Discontinued
Cat# ALS12028
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