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>   home   >   Products   >   Primary Antibodies   >   PMS2 Antibody (aa623-639, clone 163C1251)   

PMS2 Antibody (aa623-639, clone 163C1251)

Mouse Monoclonal Antibody

     
  • WB - PMS2 Antibody (aa623-639, clone 163C1251) ALS12040
    Western blot of PMS2 using antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.
  • IHC - PMS2 Antibody (aa623-639, clone 163C1251) ALS12040
    Anti-PMS2 antibody IHC of human breast.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P54278
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 163C1251
Calculated MW 96kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 107984056;5395
Other Names Mismatch repair endonuclease PMS2, 3.1.-.-, DNA mismatch repair protein PMS2, PMS1 protein homolog 2, PMS2, PMSL2
Target/Specificity A synthetic peptide corresponding to amino acids 623-639 SSLAKRIKQLHHEAQQS of human PMS2; GenBank No. NP_000526.1.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsPMS2 Antibody (aa623-639, clone 163C1251) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PMS2 (HGNC:9122)
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Cellular Location Nucleus.
Citations (0)

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Background

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

References

Nicolaides N.C.,et al.Nature 371:75-80(1994).
Tabata Y.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
Bronner C.E.,et al.Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).

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$ 395.00
Cat# ALS12040
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