MBD3 Antibody (aa215-230, clone 106B691)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
---|---|
Primary Accession | O95983 |
Reactivity | Human, Pig, Chicken, Horse, Xenopus, Bovine, Dog |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 106B691 |
Calculated MW | 33kDa |
Dilution | IHC-P (10 µg/ml), WB (2 µg/ml), |
Gene ID | 53615 |
---|---|
Other Names | Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3 |
Target/Specificity | Amino acids215-230 (CKAFMVTDEDIRKQEE) of humanMBD3 protein were used as the immunogen. |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | MBD3 Antibody (aa215-230, clone 106B691) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MBD3 |
---|---|
Function | Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:12124384, PubMed:16428440, PubMed:28977666). Acts as transcriptional repressor and plays a role in gene silencing (PubMed:10947852, PubMed:18644863). Does not bind to methylated DNA by itself (PubMed:12124384, PubMed:16428440). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases. |
Cellular Location | Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
References
Hendrich B.,et al.Mol. Cell. Biol. 18:6538-6547(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Tatematsu K.,et al.Genes Cells 5:677-688(2000).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.