|Application ||WB, IHC-P|
|Reactivity||Human, Pig, Chicken, Horse, Xenopus, Bovine, Dog|
|Dilution||IHC-P (10 µg/ml), WB (2 µg/ml),|
|Other Names||Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3|
|Target/Specificity||Amino acids215-230 (CKAFMVTDEDIRKQEE) of humanMBD3 protein were used as the immunogen.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||MBD3 Antibody (aa215-230, clone 106B691) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.|
|Cellular Location||Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes|
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Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
Hendrich B.,et al.Mol. Cell. Biol. 18:6538-6547(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Tatematsu K.,et al.Genes Cells 5:677-688(2000).
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