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>   home   >   Products   >   Primary Antibodies   >   Microbiology   >   IFIH1 / MDA5 Antibody (C-Terminus)   

IFIH1 / MDA5 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IHC - IFIH1 / MDA5 Antibody (C-Terminus) ALS12137
    Anti-MDA5 antibody IHC of human pancreas.
  • IF - IFIH1 / MDA5 Antibody (C-Terminus) ALS12137
    Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/ml.
  • WB - IFIH1 / MDA5 Antibody (C-Terminus) ALS12137
    Western blot of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
  • IHC - IFIH1 / MDA5 Antibody (C-Terminus) ALS12137
    Anti-MDA5 antibody IHC of mouse kidney.
  • IHC - IFIH1 / MDA5 Antibody (C-Terminus) ALS12137
    Anti-MDA5 antibody IHC of mouse pancreas.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession Q9BYX4
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 117kDa
Dilution IHC-P (5 µg/ml), WB (4 µg/ml),
Additional Information
Gene ID 64135
Other Names Interferon-induced helicase C domain-containing protein 1, 3.6.4.13, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116, IFIH1, MDA5, RH116
Target/Specificity 16 amino acid peptide from near the carboxy terminus of human MDA5.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsIFIH1 / MDA5 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFIH1
Synonyms MDA5, RH116
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
Cellular Location Cytoplasm. Nucleus. Note=May be found in the nucleus, during apoptosis
Tissue Location Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung
Research Areas
Citations (0)

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Background

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.

References

Kang D.-C.,et al.Proc. Natl. Acad. Sci. U.S.A. 99:637-642(2002).
Cocude C.,et al.J. Gen. Virol. 84:3215-3225(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kovacsovics M.,et al.Curr. Biol. 12:838-843(2002).
Kovacsovics M.,et al.Curr. Biol. 12:1633-1633(2002).

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$ 395.00
Cat# ALS12137
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