|Application ||WB, IHC-P, ICC|
|Reactivity||Human, Mouse, Rat, Hamster, Horse, Drosophila|
|Dilution||IHC-P (5 µg/ml), WB (1:500-1:1000),|
|Other Names||Cytochrome c, CYCS, CYC|
|Target/Specificity||Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.|
|Reconstitution & Storage||+4°C or -20°C, Avoid repeated freezing and thawing.|
|Precautions||CYCS / Cytochrome c Antibody (aa1-80, clone 7H8.2C12) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.|
|Cellular Location||Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane|
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Provided below are standard protocols that you may find useful for product applications.
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Evans M.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:9625-9629(1988).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Hillier L.W.,et al.Nature 424:157-164(2003).
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