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Apg7 / ATG7 Antibody (N-Terminus)

Rabbit Polyclonal Antibody

     
  • ICC - Apg7 / ATG7 Antibody (N-Terminus) ALS12166
    Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.
  • IF - Apg7 / ATG7 Antibody (N-Terminus) ALS12166
    Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.
  • WB - Apg7 / ATG7 Antibody (N-Terminus) ALS12166
    Western blot of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession O95352
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 78kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 10533
Other Names Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L
Target/Specificity 17 amino acid peptide from near the amino terminus of human APG7.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsApg7 / ATG7 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG7
Synonyms APG7L
Function E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Cellular Location Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
Tissue Location Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Research Areas
Citations (0)

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Background

E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.

References

Yuan W.,et al.Mol. Biol. Cell 10:1353-1366(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Tanida I.,et al.J. Biol. Chem. 276:1701-1706(2001).

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$ 395.00
Cat# ALS12166
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