|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat, Hamster|
|Dilution||ELISA (1:32000), IHC-P (2.5 µg/ml), WB (1:32000)|
|Other Names||Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1, FHL1, SLIM1|
|Target/Specificity||Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_00144 ...|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||SLIM / FHL1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May have an involvement in muscle development or hypertrophy.|
|Cellular Location||Isoform 1: Cytoplasm. Isoform 2: Nucleus. Cytoplasm, cytosol. Note=Predominantly nuclear in myoblasts but is cytosolic in differentiated myotubes|
|Tissue Location||Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
May have an involvement in muscle development or hypertrophy.
Morgan M.J.,et al.Biochem. Biophys. Res. Commun. 225:632-638(1996).
Morgan M.J.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Lee S.M.Y.,et al.Gene 216:163-170(1998).
Greene W.K.,et al.Gene 232:203-207(1999).
Lee S.M.Y.,et al.Gene 237:253-263(1999).
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