|Application ||WB, IHC-P, IF, ICC|
|Reactivity||Human, Mouse, Rat|
|Dilution||IHC-P (5 µg/ml)|
|Other Names||Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB|
|Target/Specificity||16 amino acid peptide from near the amino-terminus of human b-actin|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||ACTB / Beta Actin Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.|
|Cellular Location||Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs|
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Provided below are standard protocols that you may find useful for product applications.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Ponte P.,et al.Nucleic Acids Res. 12:1687-1696(1984).
Nakajima-Iijima S.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985).
Ohmori H.,et al.J. Cell Biol. 116:933-941(1992).
Hillier L.W.,et al.Nature 424:157-164(2003).
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).
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