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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GNAI1 / Gi Antibody (clone R4.5)   

GNAI1 / Gi Antibody (clone R4.5)

Mouse Monoclonal Antibody

     
  • IHC - GNAI1 / Gi Antibody (clone R4.5) ALS12313
    Anti-GNAI1 antibody IHC of human pancreas.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P63096
Reactivity Human, Mouse, Rat, Bovine, Guinea Pig
Host Mouse
Clonality Monoclonal
Clone Names R4.5
Calculated MW 40kDa
Dilution IHC-P (1:50)
Additional Information
Gene ID 2770
Other Names Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1
Target/Specificity Recognizes Rat Gai1. Does not cross-react with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species cross-reactivity: mouse, bovine, guinea pig and human.
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsGNAI1 / Gi Antibody (clone R4.5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNAI1
Function Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P10824}. Cytoplasm. Cell membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P10824}; Cytoplasmic side {ECO:0000250|UniProtKB:P10824}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Membrane {ECO:0000250|UniProtKB:P63096}; Lipid-anchor {ECO:0000250|UniProtKB:P63096}. Note=Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody (PubMed:17635935). Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:P10824, ECO:0000269|PubMed:17635935}
Volume 125 µl
Research Areas
Citations (0)

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Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.

References

Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Yu W.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS12313
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