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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   TRIM5 Antibody   

TRIM5 Antibody

Goat Polyclonal Antibody

     
  • IHC - TRIM5 Antibody ALS12320
    Anti-TRIM5 antibody IHC of human uterus.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9C035
Reactivity Human, Monkey
Host Goat
Clonality Polyclonal
Calculated MW 56kDa
Dilution ELISA (1:16000), IHC-P (5 µg/ml), WB (1-3 µg/ml)
Additional Information
Gene ID 85363
Other Names Tripartite motif-containing protein 5, 6.3.2.-, RING finger protein 88, TRIM5, RNF88
Target/Specificity Human TRIM5. This antibody is expected to recognise all three human isoforms of this protein.
Reconstitution & Storage Store at -20°C. Minimize freezing and thawing.
PrecautionsTRIM5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM5
Synonyms RNF88
Function Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF- kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV) (PubMed:17156811). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 (PubMed:25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction (PubMed:25127057).
Cellular Location Cytoplasm. Cytoplasm, P-body. Nucleus {ECO:0000250|UniProtKB:Q0PF16}. Note=Predominantly cytoplasmic, and is only occasionally and partially localized in the nucleus (PubMed:25127057). Closely associates with proteasomal subunits in cytoplasmic bodies (By similarity). Colocalizes with SQSTM1 in cytoplasmic bodies (By similarity). {ECO:0000250|UniProtKB:Q0PF16, ECO:0000269|PubMed:25127057}
Research Areas
Citations (0)

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Background

Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF- kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV).

References

Reymond A.,et al.EMBO J. 20:2140-2151(2001).
Yap M.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:10786-10791(2004).
Sawyer S.L.,et al.Curr. Biol. 16:95-100(2006).
Yamauchi K.,et al.FEBS J. 275:1540-1555(2008).
Battivelli E.,et al.J. Virol. 85:7828-7835(2011).

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$ 395.00
Cat# ALS12320
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