SMUG1 Antibody (N-Terminus)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q53HV7 |
Reactivity | Human, Monkey |
Host | Goat |
Clonality | Polyclonal |
Calculated MW | 30kDa |
Dilution | ELISA (1:32000), IHC-P (2.5 µg/ml), WB (0.5-2 µg/ml) |
Gene ID | 23583 |
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Other Names | Single-strand selective monofunctional uracil DNA glycosylase, 3.2.2.-, SMUG1 |
Target/Specificity | Human SMUG1. |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | SMUG1 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SMUG1 |
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Function | Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5- formylcytosine), nor other oxidized bases. The activity is damage- specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. |
Cellular Location | Nucleus |
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Background
Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA.
References
Haushalter K.A.,et al.Curr. Biol. 9:174-185(1999).
Masaoka A.,et al.Biochemistry 42:5003-5012(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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