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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   NOD2 / CARD15 Antibody (C-Terminus)   

NOD2 / CARD15 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IF - NOD2 / CARD15 Antibody (C-Terminus) ALS12421
    Immunofluorescence of NOD2 in HeLa cells with NOD2 antibody at 20 ug/ml.
  • IHC - NOD2 / CARD15 Antibody (C-Terminus) ALS12421
    Anti-NOD2 antibody IHC of human small intestine.
  • ICC - NOD2 / CARD15 Antibody (C-Terminus) ALS12421
    Immunocytochemistry of NOD2 in HeLa cells with NOD2 antibody at 10 µg/ml.
  • WB - NOD2 / CARD15 Antibody (C-Terminus) ALS12421
    Western blot of NOD2 in HeLa cell lysate with NOD2 antibody at (A) 2 and (B) 4 ug/ml.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E
Primary Accession Q9HC29
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 115kDa
Dilution ICC (10 µg/ml), IHC-P (3 µg/ml), WB (2-4 µg/ml)
Additional Information
Gene ID 64127
Other Names Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15, Inflammatory bowel disease protein 1, NOD2, CARD15, IBD1
Target/Specificity synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsNOD2 / CARD15 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOD2
Synonyms CARD15, IBD1
Function Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561).
Cellular Location Cytoplasm. Membrane. Basolateral cell membrane
Tissue Location Monocytes-specific.
Research Areas
Citations (0)

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Background

Recognizes muramyl dipeptide (MDP) constituents of bacterial peptidoglycans and plays a key role in gastrointestinal immunity: upon stimulation, binds the proximal adapter receptor- interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling, leading to activate the transcription of hundreds of genes involved in immune response.

References

Ogura Y.,et al.J. Biol. Chem. 276:4812-4818(2001).
Hugot J.-P.,et al.Nature 411:599-603(2001).
Kramer M.,et al.BMC Res. Notes 3:224-224(2010).
Tao M.,et al.Curr. Biol. 19:1255-1263(2009).
Zhao Y.,et al.Inflamm. Bowel Dis. 18:603-612(2012).

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$ 395.00
Cat# ALS12421
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