|Application ||WB, IHC-P, IF, ICC, E|
|Dilution||ICC (10 µg/ml), IHC-P (3 µg/ml), WB (2-4 µg/ml)|
|Other Names||Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15, Inflammatory bowel disease protein 1, NOD2, CARD15, IBD1|
|Target/Specificity||synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||NOD2 / CARD15 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561). Component of an autophagy-mediated antibacterial pathway together with ATG16L1 (PubMed:20637199). Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon (PubMed:19701189).|
|Cellular Location||Cytoplasm Membrane. Mitochondrion. Basolateral cell membrane|
|Tissue Location||Expressed in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes muramyl dipeptide (MDP) constituents of bacterial peptidoglycans and plays a key role in gastrointestinal immunity: upon stimulation, binds the proximal adapter receptor- interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling, leading to activate the transcription of hundreds of genes involved in immune response.
Ogura Y.,et al.J. Biol. Chem. 276:4812-4818(2001).
Hugot J.-P.,et al.Nature 411:599-603(2001).
Kramer M.,et al.BMC Res. Notes 3:224-224(2010).
Tao M.,et al.Curr. Biol. 19:1255-1263(2009).
Zhao Y.,et al.Inflamm. Bowel Dis. 18:603-612(2012).
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