|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:128000), IHC-P (3.75 µg/ml), WB (0.1-0.3 µg/ml)|
|Other Names||TBC1 domain family member 4, Akt substrate of 160 kDa, AS160, TBC1D4, AS160, KIAA0603|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||TBC1D4 / AS160 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.|
|Cellular Location||Cytoplasm. Note=Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells|
|Tissue Location||Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.|
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Provided below are standard protocols that you may find useful for product applications.
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Baus D.,et al.Cell. Signal. 20:2237-2246(2008).
Ishibashi K.,et al.Genes Cells 14:41-52(2009).
Nagase T.,et al.DNA Res. 5:31-39(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham A.,et al.Nature 428:522-528(2004).
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