|Application ||WB, IHC-P, IF, IP|
|Dilution||IHC-P (1:100), WB (1:500),|
|Other Names||Regulator of chromosome condensation, Cell cycle regulatory protein, Chromosome condensation protein 1, RCC1, CHC1|
|Target/Specificity||Reacts with human & Xenopus RanGEF|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||RCC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double- stranded DNA (PubMed:17435751, PubMed:18762580).|
|Cellular Location||Nucleus. Chromosome Cytoplasm Note=Predominantly nuclear in interphase cells (PubMed:12194828) Binds to mitotic chromosomes (PubMed:12194828, PubMed:17435751, PubMed:20668449).|
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Provided below are standard protocols that you may find useful for product applications.
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo- cytoplasmic transport, mitosis and nuclear-envelope assembly.
Ohtsubo M.,et al.Genes Dev. 1:585-593(1987).
Furuno N.,et al.Genomics 11:459-461(1991).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Miyabashira J.,et al.J. Cell Sci. 107:2203-2208(1994).
Bischoff F.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:8617-8621(1990).
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