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MIF Antibody (clone 4E4)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P14174 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4E4 |
| Calculated MW | 12kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:2000), |
| Gene ID | 4282 |
|---|---|
| Other Names | Macrophage migration inhibitory factor, MIF, 5.3.2.1, Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, 5.3.3.12, Phenylpyruvate tautomerase, MIF, GLIF, MMIF |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Precautions | MIF Antibody (clone 4E4) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MIF {ECO:0000303|PubMed:2552447, ECO:0000312|HGNC:HGNC:7097} |
|---|---|
| Function | Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494). |
| Cellular Location | Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
References
Weiser W.Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7522-7526(1989).
Mikayama T.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:10056-10060(1993).
Bernhagen J.,et al.Biochemistry 33:14144-14155(1994).
Paralkar V.,et al.Genomics 19:48-51(1994).
Shan Z.X.,et al.Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
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