|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:128000), IHC-P (4 µg/ml), WB (1-3 µg/ml)|
|Other Names||Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||CD14 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).|
|Cellular Location||Cell membrane; Lipid- anchor, GPI-anchor. Secreted Membrane raft. Golgi apparatus. Note=Secreted forms may arise by cleavage of the GPI anchor.|
|Tissue Location||Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.|
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Provided below are standard protocols that you may find useful for product applications.
In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Haziot A.,et al.J. Immunol. 141:547-552(1988).
Ferrero E.,et al.Nucleic Acids Res. 16:4173-4173(1988).
Setoguchi M.,et al.Biochim. Biophys. Acta 1008:213-222(1989).
Simmons D.L.,et al.Blood 73:284-289(1989).
Long J.Y.,et al.Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 25:377-378(1998).
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