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>   home   >   Products   >   Primary Antibodies   >   XBP1 Antibody (aa2-160, clone 9B7E5)   

XBP1 Antibody (aa2-160, clone 9B7E5)

Mouse Monoclonal Antibody

     
  • ICC - XBP1 Antibody (aa2-160, clone 9B7E5) ALS12665
    Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 ug/ml.
  • IF - XBP1 Antibody (aa2-160, clone 9B7E5) ALS12665
    Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 2 ug/ml.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E
Primary Accession P17861
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Clone Names 9B7E5
Calculated MW 29kDa
Dilution IHC-P (10 µg/ml), WB (0.5-1 µg/ml),
Additional Information
Gene ID 7494
Other Names X-box-binding protein 1 {ECO:0000303|PubMed:2321018, ECO:0000312|HGNC:HGNC:12801}, XBP-1, Tax-responsive element-binding protein 5, TREB-5, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, luminal form, XBP1 (HGNC:12801)
Reconstitution & Storage Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsXBP1 Antibody (aa2-160, clone 9B7E5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name XBP1 (HGNC:12801)
Function Functions as a transcription factor during endoplasmic reticulum (ER) stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development, and the development of secretory tissues such as exocrine pancreas and salivary gland (By similarity). Involved in terminal differentiation of B lymphocytes to plasma cells and production of immunoglobulins (PubMed:11460154). Modulates the cellular response to ER stress in a PIK3R-dependent manner (PubMed:20348923). Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (PubMed:8349596). Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention (By similarity).
Cellular Location Endoplasmic reticulum. Note=Colocalizes with ERN1 and KDR in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610). Isoform 2: Nucleus {ECO:0000250|UniProtKB:O35426, ECO:0000269|PubMed:16461360, ECO:0000269|PubMed:19394296, ECO:0000269|PubMed:20348923, ECO:0000269|PubMed:20955178}. Cytoplasm {ECO:0000250|UniProtKB:O35426}. Note=Localizes predominantly in the nucleus. Colocalizes in the nucleus with SIRT1. Translocates into the nucleus in a PIK3R-, ER stress-induced- and/or insulin- dependent manner (By similarity). {ECO:0000250|UniProtKB:O35426}
Tissue Location Expressed in plasma cells in rheumatoid synovium (PubMed:11460154). Over-expressed in primary breast cancer and metastatic breast cancer cells (PubMed:25280941) Isoform 1 and isoform 2 are expressed at higher level in proliferating as compared to confluent quiescent endothelial cells (PubMed:19416856).
Citations (0)

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Background

Functions as a transcription factor during endoplasmic reticulum (ER) stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development, and the development of secretory tissues such as exocrine pancreas and salivary gland (By similarity). Involved in terminal differentiation of B lymphocytes to plasma cells and production of immunoglobulins (PubMed:11460154). Modulates the cellular response to ER stress in a PIK3R-dependent manner (PubMed:20348923). Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (PubMed:8349596). Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention (By similarity).

References

Liou H.-C.,et al.Science 247:1581-1584(1990).
Yoshimura T.,et al.EMBO J. 9:2537-2542(1990).
Ponath P.D.,et al.J. Biol. Chem. 268:17074-17082(1993).
Yoshida H.,et al.Cell 107:881-891(2001).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).

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$ 395.00
Cat# ALS12665
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