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APG12 / ATG12 Antibody (Internal)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | O94817 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 15kDa |
| Dilution | IHC-P (5 µg/ml), WB (0.5-1 µg/ml), |
| Gene ID | 9140 |
|---|---|
| Other Names | Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12, APG12, APG12L |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
| Precautions | APG12 / ATG12 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATG12 (HGNC:588) |
|---|---|
| Synonyms | APG12, APG12L |
| Function | Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. |
| Cellular Location | Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12- ATG5 conjugate to the autolysosomal membrane |
| Tissue Location | Ubiquitous.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.
References
Mizushima N.,et al.J. Biol. Chem. 273:33889-33892(1998).
Schmutz J.,et al.Nature 431:268-274(2004).
Tanida I.,et al.J. Biol. Chem. 276:1701-1706(2001).
Tanida I.,et al.Biochem. Biophys. Res. Commun. 296:1164-1170(2002).
Tanida I.,et al.J. Biol. Chem. 277:13739-13744(2002).
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