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APG12 / ATG12 Antibody (Internal)

Rabbit Polyclonal Antibody

     
  • WB - APG12 / ATG12 Antibody (Internal) ALS12695
    Western blot of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the...
  • IF - APG12 / ATG12 Antibody (Internal) ALS12695
    Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/ml.
  • IHC - APG12 / ATG12 Antibody (Internal) ALS12695
    Anti-ATG12 antibody IHC of human adrenal.
  • IHC - APG12 / ATG12 Antibody (Internal) ALS12695
    Anti-ATG12 antibody IHC of human prostate.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O94817
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 15kDa
Dilution IHC-P (5 µg/ml), WB (0.5-1 µg/ml),
Additional Information
Gene ID 9140
Other Names Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12, APG12, APG12L
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsAPG12 / ATG12 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG12
Synonyms APG12, APG12L
Function Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.
Cellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Background

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.

References

Mizushima N.,et al.J. Biol. Chem. 273:33889-33892(1998).
Schmutz J.,et al.Nature 431:268-274(2004).
Tanida I.,et al.J. Biol. Chem. 276:1701-1706(2001).
Tanida I.,et al.Biochem. Biophys. Res. Commun. 296:1164-1170(2002).
Tanida I.,et al.J. Biol. Chem. 277:13739-13744(2002).

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$ 395.00
Cat# ALS12695
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