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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   PARP1 Antibody (clone A6.4.12)   

PARP1 Antibody (clone A6.4.12)

Mouse Monoclonal Antibody

     
  • IHC - PARP1 Antibody (clone A6.4.12) ALS12822
    Anti-PARP antibody IHC of human placenta.
  • IHC - PARP1 Antibody (clone A6.4.12) ALS12822
    Anti-PARP antibody IHC of human spleen.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E, IP, IHC-Fr
Primary Accession P09874
Reactivity Human, Mouse, Rat, Hamster, Xenopus, Drosophila
Host Mouse
Clonality Monoclonal
Clone Names A6.4.12
Calculated MW 113kDa
Dilution ELISA (1:1000-1:5000), IHC-P (10 µg/ml),
Additional Information
Gene ID 142
Other Names Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL
Target/Specificity Recognizes poly (ADP-ribose) polymerase (PARP), a 116kD nuclear enzyme that has been shown to be cleaved during apoptosis
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsPARP1 Antibody (clone A6.4.12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PARP1
Synonyms ADPRT, PPOL
Function Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).
Cellular Location Nucleus. Nucleus, nucleolus. Note=Localizes at sites of DNA damage
Research Areas
Citations ( 0 )

Background

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.

References

Uchida K.,et al.Biochem. Biophys. Res. Commun. 148:617-622(1987).
Kurosaki T.,et al.J. Biol. Chem. 262:15990-15997(1987).
Cherney B.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:8370-8374(1987).
Auer B.,et al.DNA 8:575-580(1989).
Gregory S.G.,et al.Nature 441:315-321(2006).

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$ 395.00
Cat# ALS12822
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