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AMACR Antibody (clone 2A10F3)

Mouse Monoclonal Antibody

     
  • IF - AMACR Antibody (clone 2A10F3) ALS12834
    Confocal immunofluorescence of LNCaP cells using AMACR mouse monoclonal antibody (green).
    detail
  • IHC - AMACR Antibody (clone 2A10F3) ALS12834
    Anti-AMACR antibody IHC of human brain, cerebellum.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9UHK6
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2A10F3
Calculated MW 42kDa
Dilution ELISA (1:10000), IF (1:200-1:1000), IHC-P (1:200), WB (1:500-1:2000) ,
Additional Information
Gene ID 23600
Other Names Alpha-methylacyl-CoA racemase, 5.1.99.4, 2-methylacyl-CoA racemase, AMACR
Target/Specificity Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3)
PrecautionsAMACR Antibody (clone 2A10F3) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMACR
Function Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2- (4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).
Cellular Location Peroxisome. Mitochondrion
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.

References

Amery L.,et al.J. Lipid Res. 41:1752-1759(2000).
Ferdinandusse S.,et al.Nat. Genet. 24:188-191(2000).
Mubiru J.N.,et al.Prostate 65:117-123(2005).
Albers C.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Ouyang B.,et al.Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.

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$ 467.50
Cat# ALS12834
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