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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   MLH1 Antibody   

MLH1 Antibody

Mouse Monoclonal Antibody

     
  • WB - MLH1 Antibody ALS12841
    Western blot using MLH1 mouse monoclonal antibody against HeLa (1), MCF-7 (2) and A549 (3),...
  • IF - MLH1 Antibody ALS12841
    Confocal immunofluorescence of HeLa cells using MLH1 mouse monoclonal antibody (green), showing...
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P40692
Reactivity Human, Monkey
Host Mouse
Clonality Monoclonal
Calculated MW 85kDa
Dilution ELISA (1:10000), IF (1:200-1:1000), IHC-P (1:200), WB (1:500-1:2000) ,
Additional Information
Gene ID 4292
Other Names DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1, COCA2
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsMLH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MLH1
Synonyms COCA2
Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Cellular Location Nucleus
Tissue Location Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart
Research Areas
Citations (0)

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Background

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

References

Bronner C.E.,et al.Nature 368:258-261(1994).
Papadopoulos N.,et al.Science 263:1625-1629(1994).
Kolodner R.D.,et al.Cancer Res. 55:242-248(1995).
Han H.-J.,et al.Hum. Mol. Genet. 4:237-242(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS12841
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