|Application ||WB, IHC-P, E, IP|
|Dilution||ELISA (1:2000-1:10000), IHC-P (5 µg/ml), WB (1:2000-1:10000)|
|Other Names||Apolipoprotein B-100, Apo B-100, Apolipoprotein B-48, Apo B-48, APOB|
|Target/Specificity||Typically less than 1% cross reactivity againstother types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, ...|
|Reconstitution & Storage||Store vial at 4 C prior to opening. This product is stable 4 C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 C or below.|
|Precautions||APOB / Apolipoprotein B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.|
|Cellular Location||Cytoplasm. Secreted|
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Provided below are standard protocols that you may find useful for product applications.
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Knott T.C.,et al.Nucleic Acids Res. 14:7501-7503(1986).
Ludwig E.H.,et al.DNA 6:363-372(1987).
Chen S.-H.,et al.J. Biol. Chem. 261:12918-12921(1986).
Law S.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:8142-8146(1986).
Cladaras C.,et al.EMBO J. 5:3495-3507(1986).
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