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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   AIFM1 / AIF / PDCD8 Antibody (aa593-606)   

AIFM1 / AIF / PDCD8 Antibody (aa593-606)

Rabbit Polyclonal Antibody

     
  • ICC - AIFM1 / AIF / PDCD8 Antibody (aa593-606) ALS12954
    Immunocytochemistry of AIF in K562 cells with AIF antibody at 5 ug/ml.
  • WB - AIFM1 / AIF / PDCD8 Antibody (aa593-606) ALS12954
    Western blot of AIF in K562 cell lysate (A), mouse (B), and rat (C) liver tissue lysates with...
  • IHC - AIFM1 / AIF / PDCD8 Antibody (aa593-606) ALS12954
    Anti-AIFM1 / AIF antibody IHC of human kidney.
  • IHC - AIFM1 / AIF / PDCD8 Antibody (aa593-606) ALS12954
    Anti-AIFM1 / AIF antibody IHC of human liver.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC, E
Primary Accession O95831
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Horse, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 67kDa
Dilution IHC-P (5 µg/ml), WB (0.25-1 µg/ml),
Additional Information
Gene ID 9131
Other Names Apoptosis-inducing factor 1, mitochondrial, 1.1.1.-, Programmed cell death protein 8, AIFM1, AIF, PDCD8
Target/Specificity peptide (KDGEQHEDLNEVAK) corresponding to amino acids 593 to 606 of human AIF. This sequence is identical to those of mouse and rat AIF.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsAIFM1 / AIF / PDCD8 Antibody (aa593-606) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AIFM1
Synonyms AIF, PDCD8
Function Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Cellular Location Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region Isoform 5: Cytoplasm
Tissue Location Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.
Research Areas
Citations (0)

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Background

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

References

Susin S.A.,et al.Nature 397:441-446(1999).
Delettre C.,et al.J. Biol. Chem. 281:6413-6427(2006).
Delettre C.,et al.J. Biol. Chem. 281:18507-18518(2006).
Rhodes S.,et al.Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS12954
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