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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AAK1 Antibody (C-Terminus)   

AAK1 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • WB - AAK1 Antibody (C-Terminus) ALS12989
    Western blot of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/ml.
  • IHC - AAK1 Antibody (C-Terminus) ALS12989
    Anti-AAK1 antibody IHC of human kidney.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q2M2I8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 104kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 22848
Other Names AP2-associated protein kinase 1, 2.7.11.1, Adaptor-associated kinase 1, AAK1, KIAA1048
Target/Specificity Immunogen is specific for Isoform 1.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsAAK1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AAK1
Synonyms KIAA1048
Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Cellular Location Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity).
Tissue Location Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain
Research Areas
Citations (0)

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Background

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

References

Henderson D.M.,et al.Mol. Biol. Cell 18:2698-2706(2007).
Kikuno R.,et al.DNA Res. 6:197-205(1999).
Hillier L.W.,et al.Nature 434:724-731(2005).
Conner S.D.,et al.J. Cell Biol. 162:773-779(2003).
Olsen J.V.,et al.Cell 127:635-648(2006).

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$ 395.00
Cat# ALS12989
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